Natural Science and Discovery 2021-07-24T12:38:53+03:00 Bektas Tepe, Ph.D. Open Journal Systems <p><strong>Natural Science and Discovery</strong> (2149-6307) is international open access, peer-reviewed scientific research journal that provides rapid publication of articles in all disciplines of Life science such as Physics, Mathematics, Chemistry, Biology, Engineering, Nanotechnology</p> Biosorption of Cu2+ by Pseudomonas putida Immobilized on Loofa Sponge (Luffa cylindrica L.) 2021-07-12T10:40:21+03:00 Arzuhan SIHOGLU TEPE Emine KACMAZOGLU H. Nursevin OZTOP Rana TASKIN <p>Cu<sup>2+</sup> contaminated areas pose serious health risk for the living organisms. In this study, the biosorption of Cu2+ by <em>Pseudomonas putida</em> immobilized on loofa sponge (<em>Luffa cylindrica</em> L.) was investigated. Effects of the particle size of loofa sponge, initial pH, temperature, initial Cu2+ concentration and the stirring speed on the adsorption of Cu<sup>2+</sup> were examined. Optimum conditions were determined as follows: loofa sponge particle size is 0.42 - 0.85 mm, initial pH is 5.0, temperature is 30 °C, initial Cu<sup>2+</sup> concentration is 25 mg/l, and stirring speed is 130 rpm. According to the results of kinetic calculations, Qmax and ro values were determined as 0.394 mg/g and 0.077 mg/g.min for <em>P. putida</em> immobilized on loofa sponge, respectively, while they were found to be 0.096 mg/g and 0.052 mg/g.min for the loofa sponge only. It is thought that Cu<sup>2+</sup> can be removed effectively from the wastewaters by using <em>P. putida</em> immobilized on loofa sponge.</p> 2021-06-30T00:00:00+03:00 Copyright (c) 2021 Arzuhan SIHOGLU TEPE, Emine KACMAZOGLU, H. Nursevin OZTOP, Rana TASKIN