Karyotype Analysis of Tulipa pulchella (Liliaceae) (Fenzl ex Regel) Baker

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Published: Dec 15, 2016
DOI: https://doi.org/10.20863/nsd.v2i4.45
Keywords:
Tulipa pulchella Chromosome Number Karyotype

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Yasar Kiran
Firat University, Science Faculty, Dept. of Biology, Elazig
Gulden Dogan
Firat University, Science Faculty, Dept. of Biology, Elazig
Zeynep Demirkan
Firat University, Science Faculty, Dept. of Biology, Elazig

Abstract

Objective:  In this study the somatic chromosome numbers and detailed morphometric properties of Tulipa pulchella (Fenzl ex Regel) Baker was examined.


Material and Methods:  The seeds were germinated on moist filter paper in petri dishes at 25°C. Actively growing root tips were pretreated with 0.05 M colchicine for 6 h at room temperature. Then, the root tips were fixed with acetic alcohol (1:3 glacial acetic acid–absolute ethanol) for at least 24 h at 4°C, hydrolyzed in 1 N HCl at 60°C for 1-2 min, then rinsed in tap water for 3–5 min. Finally, they were stained in Feulgen for 1 h and mounted in 45% acetic acid. Digital microphotographs from at least five well-spread metaphase plates were taken using an Olympus BX51 microscope X40 objective and were recorded with an Olympus Camedia C-4000 digital camera.


Results:  The chromosome number of Tulipa pulchella is determined as 2n = 24 for this taxon. The karyotype consists of 8 submedian region (sm) and 4 subterminal (st) chromosomes. The metaphase chromosome length ranges from 9.35 to 16.48 μm, longest to shortest chromosome ratio was 1.9:4.1, total karyotype length (TKL) was 150.99 um and the karyotype symmetry was type 1A.


Conclusion: The basic chromosome number of Tulipa pulchella is x=12. The majority of tulip species and cultivars is diploid (2n=2x=24), however, encounters to triploids (2n=3x=36), tetraploids (2n=4x=48) and even some pentaploids (2n=5x=60) and hexaploid (2n=6x=72). Identifying the chromosome number of this species in this study would provide a base for biosystematic studies.


 

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How to Cite
Kiran, Y., Dogan, G., & Demirkan, Z. (2016). Karyotype Analysis of Tulipa pulchella (Liliaceae) (Fenzl ex Regel) Baker. Natural Science and Discovery, 2(4), 62–67. https://doi.org/10.20863/nsd.v2i4.45
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Vol. 2 No. 4 (2016): Natural Science and Discovery
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Research Article
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