Seroprevalence of Toxoplasmosis in Free Range chickens in Tabriz area of Iran by using ELISA test

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Fathollah Muhammad
Yagoob Garedaghi


Objective: As consumption of chicken meat may be as one of the sources of human infection, this study was undertaken to determine the prevalence of T. gondii among the free Range Chicken , by using ELISA  in Tabriz, Northwest of Iran.

Materials and Methods: One hundred blood samples were collected from free Range chickens by a cluster random sampling method and tested for toxoplasmosis by Enzyme Linked Immunosorbant Assay (ELISA). The results were analyzed by SPSS software using Chi-Square test and a value <0.05 was considered statically significant.

Results: Results revealed ,that 18% of Free-Ranging chickens positive by ELISA respectively. Although the difference observed in the percentage of T. gondii antibodies among different areas of Tabriz city, Iran, there was no significant differences P < 0.05 detected by the ELISA method.

Conclusion: Based on cultural and food habits in our area, the meat and viscera of chicken may be important sources of infection in human when consuming semi-cooked meats. Considering the high prevalence of toxoplasmosis in chickens, standards in chicken breeding, education of environmental health personnel and standardization for preparation and handling techniques are required by Health and Veterinary organizations.

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How to Cite
Muhammad, F. ., & Garedaghi, Y. (2016). Seroprevalence of Toxoplasmosis in Free Range chickens in Tabriz area of Iran by using ELISA test. Natural Science and Discovery, 2(1), 20–23. Retrieved from
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Smith JE, Reduck NR. Toxoplasma gondii strain variation and pathogenicity. In: Cary, J.W., Linz, J.E., Bhatnagar, B. (Eds), Microbial foodborne diseases: mechanisms of pathogenesis and toxin synthesis. Technomic Publishing, Lancaster, PA, 2000: 405-431.
Dubey JP, Beattie CP. Toxoplasmmosis of animal and man. CRC Press, Boca Raton, FL, 1988: 1-220.
Remington JS, Desmonts G. Toxoplasmosis. In: Remington, J.S., Klein, J.O., eds. Infectious diseases of the fetus and newborn infant. Philadelphia.
Oksenhendler E, Charreau I, Tournerie C, Azihary M, Carbon C, Aboulker JP. Toxoplasma gondii infection in advanced HIV infection. AIDS. 1994; 8(4): 483-7.
WB, 1990: 170-185.5. Dubey, J.P. (2010). Toxoplasma gondii infections in chickens (Gallus domesticus): Prevalence, clinical disease, diagnosis, and public health significance. Journal of Zoonosis and Public Health in press,57:60-73.
Sedaghat A, Ardehali SM, Sadigh M, Buxton M. The prevalence of toxoplasmosis infection in Southern Iran. Am J Trop Med Hyg. 1978; 81(10):204-7.
Assmar M, Amirkhani A, Piazak N, Hovaesian A, Koolobandi A, Etessami R. Toxoplasmosis in Iran. Results of a seroepidemiological study. Bull de la Soc Patholog Exo. 1997; 90:19-21.
Dubey JP. Advances in the life cycle of Toxoplasma gondii. Inter J Parasitol. 1998; 28:1019-1024.
Tenter AM, Heckeroth AR, Weiss LM, Toxoplasma gondii: From animal to human. Inter J Parasitol. 2000; 30:1217-58.
Devada K, Anandan R, Dubey JP, Serologic prevalence of Toxoplasma gondii in chickens in Madras. Ind J Parasitol. 1998; 84: 621-622.
El-Massey, A.; Mahdy, O. A.; El-Ghaysh, A.and Dubey, J.P., (2000). Prevalence of Toxoplasma gondii antibodies in sera of turkeys, chickens, and ducks from Egypt. J. Parasitol. 86, 627–628.
Zumbado, S.G., Miranda, J.G., Bermidez, G.O. and Chinchilla, M., (2000). Elminacion de ooquistes de Toxoplasma gondii en politos despues de una infection experimental. clencias,23,25-34.
Ghorbani M.; Gharavi M. J.and Kahnamoui A. (1990). Serological and parasitological investigations on Toxoplasma infection in domestic fowls in Iran. Iranian J Pub Health.; 19: 9-17.
Devada, K.; Anandan, R. and Dubey, J. P. (1998). Serologic prevalence of Toxoplasma gondii in chickens in Madras, India. J. Parasitol. 84, 621–622.
Rifaat, M. A.; Morsy, T. A. and Sadek, M. S. M. (1969). Toxoplasmosis in chickens and pigeons in U.A.R. (Preliminary report). J. Trop. Med. Hyg. 72, 193–194.
Hassanian, M. A.; Zayed, A. A.; Derbala, A. A. and Kutkat, M. A. (1997). Serological diagnosis of Toxoplasma gondii (Apicomplexa: Toxoplasminae) infection in laying hens. Egyptian Journal of Applied Science 12: 1–8.
Dubey, J. P.; Ruff, M. D. ; Camargo, M. E. ; Shen, S. K. ;Wilkins, G. L.; Kwok, O. C. H. and Thulliez, P. (1993). Serologic and parasitologic responses of domestic chickens after oral inoculation with Toxoplasma gondii oocysts. Am. J. Vet. Res. 54, 1668–1672.
Da Silva, D. S.; Bahia-Oliveira, L. M. G. ; Shen, S. K.; Kwok, O. C. H.; Lehmann, T. and Dubey, J. P. 2003: Prevalence of Toxoplasma gondii in chickens from an area in southern Brazil highly endemic to humans. J. Parasitol. 89, 394–396.
Dubey, J. P.,(2003) . Toxoplasma gondii isolates from free ranging chickens from the united states. journal of parasitology, 89,1060-1062.
Dubey, J. P., Graham, D. H. Dahl, E. Hilali, M. El-Ghaysh, A. Sreekumar, C. Kwok, O. C. H. Shen, S. K. and Lehmann, T. (2003). Isolation and molecular characterization of Toxoplasma gondii from chickens and ducks from Egypt. Vet. Parasitol. 114, 89–95.
Literák, I., and K. Hejlíček. "Incidence of Toxoplasma gondii in populations of domestic birds in the Czech Republic." Avian Pathology 22.2 (1993): 275-281.
Asgari, Q.; Farzaneh, A.; Kalantari, M.; Akrami Mohajeri, F.; Moazeni, M.; Zarifi, M.; Esmaeilzadeh, B.and Motazedian, MH. (2006). Seroprevalence of free-Ranging chicken toxoplasmosis in sub-urban regions of Shiraz, Iran. Int J Poult Sci.; 5: 262-264.
Lindsay, D. S.; Smith, P.C.and Blagurn, B.C.,(1994). prevalence
and Isolation of Toxoplasma gondii from wild turkey in Alabam. J Helmintholo Socity Washington.61:115-117
Gamble, H.R.; Dubey, J.P. & Lambillotte, D.N. (2005). Comparison of commercial ELISA with the modified agglutination test (MAT) for detection of Toxoplasma infection in domestic pigs. Vet. Parasitol. 182(3-4):177-181.
Beaver,P.C., Jung, R.C. and Cupp, E.W.(1990).coccidia, microsporidiay pneumocystis. In: parasitologia Clinica,2nd, edn, (salvat Mexicana, Mexico),179-184.
Biancifiori, F.; Rondini, C.; Grelloni, V. and Frescura, T. (1986).Avian toxoplasmosis: experimental infection of chicken and pigeon. Comp. Immunol. Microbiol. Infect. Dis. 9, 337–346.
Zhu, J.; Yin, J.; Xiao, Y.; Jiang, N.; Ankarlev, J.; Lindh, J. and Chen, Q. (2008). A seroepidemiological survey of Toxoplasma gondii infection in free-range and caged chickens in northeast china. Vet. Parasitol. 158, 360–363.
Asgari Q, Farzaneh A, Kalantari M, Akrami Mohajeri F, Moazeni M, Zarifi M, Esmaeilzadeh B, Motazedian MH. Seroprevalence of free-Ranging chicken toxoplasmosis in sub-urban regions of Shiraz, Iran. Int J Poult Sci. 2006; 5: 262- 264.
Zia-Ali N, Fazaeli A, Khoramizadeh M, Ajzenberg D, Dardé M, Keshavarz-Valian H, Isolation and molecular characterization of Toxoplasma gondii strains from different hosts in Iran. Parasitol Res. 2007; 101:111–115.
Garedaghi Yagoob, Identification of Immunogenic Relevant Antigens in the Excretory-secretory (ES) Products of Ascaridia galli Larvae, Advances in Environmental Biology, 5(6): 1120-1126, 2011.
Zia-Ali N, Keshavarz-Valian H, Rezaian M, Khoramizadeh M, Kazemi B, Fazaeli A, Dardé M, Molecular Characterization of Toxoplasma gondii from Bird Hosts. Iranian J Pub Health.2005; 34(3): 27-30.
Yagoob Garedaghi, Arash Khaki, Evaluation of the Effectiveness of Ethanolic Extract of Solanum Surattense against Plasmodium Berghei in Comparison with Chloroquine in Sourian Mice Using in Vivo Tests. Crescent Journal of Medical and Biological Sciences. Vol. 1, No. 3, Summer 2014, 76-79.
Yagoob Garedaghi,seyeid razi bahavarnia, Repairing Effect of Allium Cepa on Testis Degeneration Caused by Toxoplasma Gondii in The Rat. International Journal of Women’s Health and Reproduction Sciences. 2014; 2 (2):80-89.